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KMID : 0894520110150010025
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Development & Reproduction
2011 Volume.15 No. 1 p.25 ~ p.30
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No Relevance of NF- in the Transcriptional Regulation of Human Nanog Gene in Embryonic Carcinoma Cells
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Seok Hyun-Jeong
Kim Young-Eun
Park Jeong-A
Lee Young-Hee
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Abstract
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Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF- is a transcription factor involved in many biological activities. Expression and activity of NF- increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF- protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF- in the human Nanog promoter and postulated that NF- protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF- upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF- binding site suggested involvement of NF- in the negative regulation of the promoter, site-directed mutation of NF- binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF- with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF- protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.
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KEYWORD
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Human Nanog gene, Promoter, Negative regulation, NF-
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